Selecting columns and filtering rows

We’re going to learn some of the most common dplyr functions: select(), filter(), mutate(), group_by(), and summarize(). To select columns of a data frame, use select(). The first argument to this function is the data frame (metadata), and the subsequent arguments are the columns to keep.

select(metadata, sample, clade, cit, genome_size)

To choose rows, use filter():

filter(metadata, cit == "plus")

##     sample generation clade strain  cit       run genome_size
## 1   ZDB564      31500  Cit+ REL606 plus SRR098289        4.74
## 2   ZDB172      32000  Cit+ REL606 plus SRR098042        4.77
## 3   ZDB143      32500  Cit+ REL606 plus SRR098040        4.79
## 4   CZB152      33000  Cit+ REL606 plus SRR097977        4.80
## 5   CZB154      33000  Cit+ REL606 plus SRR098026        4.76
## 6    ZDB87      34000    C2 REL606 plus SRR098035        4.75
## 7    ZDB96      36000  Cit+ REL606 plus SRR098036        4.74
## 8   ZDB107      38000  Cit+ REL606 plus SRR098038        4.79
## 9 REL10979      40000  Cit+ REL606 plus SRR098029        4.78

Pipes

But what if you wanted to select and filter? There are three ways to do this: use intermediate steps, nested functions, or pipes. With the intermediate steps, you essentially create a temporary data frame and use that as input to the next function. This can clutter up your workspace with lots of objects. You can also nest functions (i.e. one function inside of another). This is handy, but can be difficult to read if too many functions are nested as the process from inside out. The last option, pipes, are a fairly recent addition to R. Pipes let you take the output of one function and send it directly to the next, which is useful when you need to many things to the same data set. Pipes in R look like %>% and are made available via the magrittr package installed as part of dplyr.

metadata %>%
  filter(cit == "plus") %>%
  select(sample, generation, clade)

##     sample generation clade
## 1   ZDB564      31500  Cit+
## 2   ZDB172      32000  Cit+
## 3   ZDB143      32500  Cit+
## 4   CZB152      33000  Cit+
## 5   CZB154      33000  Cit+
## 6    ZDB87      34000    C2
## 7    ZDB96      36000  Cit+
## 8   ZDB107      38000  Cit+
## 9 REL10979      40000  Cit+

In the above we use the pipe to send the metadata data set first through filter, to keep rows where cit was equal to ‘plus’, and then through select to keep the sample and generation and clade columns. When the data frame is being passed to the filter() and select() functions through a pipe, we don’t need to include it as an argument to these functions anymore.

If we wanted to create a new object with this smaller version of the data we could do so by assigning it a new name:

meta_citplus <- metadata %>%
  filter(cit == "plus") %>%
  select(sample, generation, clade)

meta_citplus

##     sample generation clade
## 1   ZDB564      31500  Cit+
## 2   ZDB172      32000  Cit+
## 3   ZDB143      32500  Cit+
## 4   CZB152      33000  Cit+
## 5   CZB154      33000  Cit+
## 6    ZDB87      34000    C2
## 7    ZDB96      36000  Cit+
## 8   ZDB107      38000  Cit+
## 9 REL10979      40000  Cit+

Challenge

Using pipes, subset the data to include rows where the clade is ‘Cit+’. Retain columns sample, cit, and genome_size.

Mutate

Frequently you’ll want to create new columns based on the values in existing columns, for example to do unit conversions or find the ratio of values in two columns. For this we’ll use mutate().

To create a new column of genome size in bp:

metadata %>%
  mutate(genome_bp = genome_size *1e6)

If this runs off your screen and you just want to see the first few rows, you can use a pipe to view the head() of the data (pipes work with non-dplyr functions too, as long as the dplyr or magrittr packages are loaded).

metadata %>%
  mutate(genome_bp = genome_size *1e6) %>%
  head

The row has a NA value for clade, so if we wanted to remove those we could insert a filter() in this chain:

metadata %>%
  mutate(genome_bp = genome_size *1e6) %>%
  filter(!is.na(clade)) %>%
  head

is.na() is a function that determines whether something is or is not an NA. The ! symbol negates it, so we’re asking for everything that is not an NA.

Split-apply-combine data analysis and the summarize() function

Many data analysis tasks can be approached using the “split-apply-combine” paradigm: split the data into groups, apply some analysis to each group, and then combine the results. dplyr makes this very easy through the use of the group_by() function, which splits the data into groups. When the data is grouped in this way summarize() can be used to collapse each group into a single-row summary. summarize() does this by applying an aggregating or summary function to each group. For example, if we wanted to group by citrate-using mutant status and find the number of rows of data for each status, we would do:

metadata %>%
  group_by(cit) %>%
  summarize(n())

## # A tibble: 3 x 2
##   cit     `n()`
##   <fct>   <int>
## 1 minus       9
## 2 plus        9
## 3 unknown    12

Here the summary function used was n() to find the count for each group. We can also apply many other functions to individual columns to get other summary statistics. For example, in the R base package we can use built-in functions like mean, median, min, and max. By default, all R functions operating on vectors that contains missing data will return NA. It’s a way to make sure that users know they have missing data, and make a conscious decision on how to deal with it. When dealing with simple statistics like the mean, the easiest way to ignore NA (the missing data) is to use na.rm=TRUE (rm stands for remove).

So to view mean genome_size by mutant status:

metadata %>%
  group_by(cit) %>%
  summarize(mean_size = mean(genome_size, na.rm = TRUE))

## # A tibble: 3 x 2
##   cit     mean_size
##   <fct>       <dbl>
## 1 minus        4.61
## 2 plus         4.77
## 3 unknown      4.62

You can group by multiple columns too:

metadata %>%
  group_by(cit, clade) %>%
  summarize(mean_size = mean(genome_size, na.rm = TRUE))

## # A tibble: 13 x 3
## # Groups:   cit [?]
##    cit     clade   mean_size
##    <fct>   <fct>       <dbl>
##  1 minus   C1           4.61
##  2 minus   C2           4.62
##  3 minus   C3           4.61
##  4 minus   Cit+         4.6 
##  5 plus    C2           4.75
##  6 plus    Cit+         4.77
##  7 unknown (C1,C2)      4.62
##  8 unknown C1           4.63
##  9 unknown C2           4.62
## 10 unknown C3           4.59
## 11 unknown UC           4.62
## 12 unknown unknown      4.62
## 13 unknown <NA>         4.62

Looks like for one of these clones, the clade is missing. We could then discard those rows using filter():

metadata %>%
  group_by(cit, clade) %>%
  summarize(mean_size = mean(genome_size, na.rm = TRUE)) %>%
  filter(!is.na(clade))

All of a sudden this isn’t running off the screen anymore. That’s because dplyr has changed our data.frame to a tbl_df. This is a data structure that’s very similar to a data frame; for our purposes the only difference is that it won’t automatically show tons of data going off the screen.

You can also summarize multiple variables at the same time:

metadata %>%
  group_by(cit, clade) %>%
  summarize(mean_size = mean(genome_size, na.rm = TRUE),
            min_generation = min(generation))

Handy dplyr cheatsheet

Much of this lesson was copied or adapted from Jeff Hollister’s materials